CGSC Strain#: 6755
Strain Designation: BW6159      Source of Strain: B.L. Wanner
Sex: Hfr    Point of Origin#: 68   Map Position: 68.60   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, ilvD691::Tn10, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ilvD691::Tn10-- : Insertion determined by sequencing to be within ilvD (F.W.Pons, pers.commun. Oct.2000)
• thiE1 was formerly called thi-1
• = tetracycline resistant

References:

• Wanner, B.L. 1986. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J.Mol.Biol. 191:39-58