CGSC Strain#: 4299
Strain Designation: KL231      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: leuS31(ts), thyA6, rpsL120(strR), deoC1
Strain Comments:

• leuS31(ts)-- is a temperature-sensitive mutation.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Low, B, F Gates, T Goldstein, D Soll 1971. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases. J. Bacteriol. 108(2):742-50.
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812