CGSC Strain#: 4298
Strain Designation: KL229      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: serS15(ts), thyA6, rpsL120(strR), deoC1
Strain Comments:

• serS15(ts)-- is a temperature-sensitive mutation.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Low, B, F Gates, T Goldstein, D Soll 1971. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases. J. Bacteriol. 108(2):742-50.