CGSC Strain#: 4291
Strain Designation: KL711      Source of Strain: M.C. Cappello
Sex: F' Episome/Plasmid: F143
   Plasmid Markers/Mutations: relA1
Chromosomal Markers: galK35, λ^-, pyrD34, e14-, trpC45, his-68, tyrA2, recA1, thyA33, rpsL118(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain should be maintained on medium containing thi, his, ura, trp, and lacking tyr, thy. F143 probably carries relA1.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA33-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.
• Kreuzer, KN, K McEntee, AP Geballe, NR Cozzarelli 1978. Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol. Gen. Genet. 167(2):129-37.