CGSC Strain#: 5638
Strain Designation: L48      Source of Strain: D.F. Silbert
Other Designations: LA2-89
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), gltA5, galK2(Oc), fabD89(ts), rpsL20(strR), xylA5, mtl-1, lldD1, thiE1, tfr-5
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• fabD89(ts)-- is a temperature-sensitive mutation.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Harder, M.E., I.R. Beacham, J.E. Cronan, K. Beacham, J.L. Honegger, D.F. Silbert 1972. Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc.Natl.Acad.Sci.USA 69:310
• Semple, KS, DF Silbert 1975. Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli. J. Bacteriol. 121(3):1036-46.