CGSC Strain#: 5623
Strain Designation: KMBL91      Source of Strain: P. van de Putte
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, codA1, lacY1, glnX44(AS), λ^-, pyrF101, rfbC1, thyA6, uvrD502, thiE1, deoC1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• uvrD502-- was formerly called uvrE502.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• van de Putte, P, CA van Sluis, J van Dillewijn, A Rorsch 1965. The location of genes controlling radiation sensitivity in Escherichia coli. Mutat. Res. 2(2):97-110.
• Smirnov, GB, AG Skavronskaya 1971. Location of uvr502 mutation on the chromosome of Escherichia coli K-12. Mol. Gen. Genet. 113(3):217-21.
• Smirnov, GB, EV Filkova, AG Skavronskaya 1972. The mutator property of uvr502 mutation affecting UV sensitivity of Escherichia coli. Mol. Gen. Genet. 118(1):51-6.
• Siegel, EC 1973. Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J. Bacteriol. 113(1):145-60.
• Siegel, EC 1973. Ultraviolet-sensitive mutator mutU4 of Escherichia coli inviable with polA. J. Bacteriol. 113(1):161-6.