CGSC Strain#: 5620
Strain Designation: WA2574      Source of Strain: W. Arber
Sex: F- Chromosomal Markers: leuB6(Am), fhuA21, lacY1 or Δ(cod-lacI)6, glnX44(AS), λ^-, manY455, metB1, thiE1, hsdS3, deoB20
Strain Comments:

• The paper cited describes related strains, but not 2574. This strain is r-K, m-K.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• deoB20-- E. coli 15 mutation.
• deoB20 was formerly called thyR32
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• manY455 was formerly called ptsM455
• manY455 was formerly called pel-5
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Arber, W, D Wauters-Willems 1970. Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Mol. Gen. Genet. 108(3):203-17.