CGSC Strain#: 5172
Strain Designation: H1t14      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: thr-20, leu-32, proA35, lacY1, tsx-70, glnX44(AS), gal-6, λ^-, rpsL125(strR), malT1(λ^R), xyl-7, mtlA2, ampCp-1, ampC12, purA45
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampC12-- encodes a heat-labile beta-lactamase.
• ampCp-1 was formerly called ampA1
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Burman, L.G., J.T. Park, E. Lindstrom, H.G. Boman 1973. Resistance of Escherichia coli to penicillins: identification of the structural gene for the chromosomal penicillinase. J.Bacteriol. 116:123-130