CGSC Strain#: 5158
Strain Designation: D21      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), ampCp-1
Strain Comments:

• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• strR = streptomycin resist.

References:

• Boman, HG, KG Eriksson-Grennberg, S Normark, E Matsson 1968. Resistance of Escherichia coli to penicillins. IV. Genetic study of mutants resistant to D,L-ampicillin concentrations o 100 mu-g-ml. Genet. Res. 12(2):169-85.
• Grundstrom, T., B. Jaurin, T. Edlund, S. Normark 1980. Physical mapping and expression of hybrid plasmids carrying chromosomal beta-lactamase genes of Escherichia coli K-12. J.Bacteriol. 143:1127-1134