CGSC Strain#: 5163
Strain Designation: D22      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: lpxC101, proA23, lac-28, tsx-81, trp-30, his-51, tufA1, rpsL173(strR), ampCp-1
Strain Comments:

• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• lpxC101 was formerly called envA1
• strR = streptomycin resist.

References:

• Normark, S, HG Boman, E Matsson 1969. Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics. J. Bacteriol. 97(3):1334-42.
• Fischer, E, H Wolf, K Hantke, A Parmeggiani 1977. Elongation factor Tu resistant to kirromycin in an Escherichia coli mutant altered in both tuf genes. Proc. Natl. Acad. Sci. U.S.A. 74(10):4341-5.